shuttered led fluorescence excitation source  (Lumencor Inc)

Journal: bioRxiv

Article Title: Optimization and Parallelization of Sorting by Interfacial Tension (SIFT) for High-Throughput Metabolic Cell Sorting

doi: 10.64898/2026.03.11.710714

Figure Lengend Snippet: a) Image of sorting region showing Unselected Droplet (circled in red) and Selected droplet (circled in green). b) pH of Selected and Unselected cell populations. Average pH value indicated by horizontal bar. c) Maximum cellular fluorescence intensity of BioTracker Far-red Labile Fe 2+ Live Cell Dye for the Unselected and Selected cell populations. The Selected and Unselected populations were statistically different (Welch’s t-test, (t(148.1) = −3.13, p = 0.002).

Article Snippet: On-chip images and videos were taken on an inverted fluorescence microscope (Olympus IX-51) equipped with a 4X objective, a shuttered LED fluorescence excitation source (Spectra-X light engine, Lumencor, Beaverton, OR) and a high-speed camera (VEO-410, Vision Research, Wayne, NJ).

Techniques: Fluorescence

Journal: Analytical chemistry

Article Title: Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations based on Single-Cell Glycolysis

doi: 10.1021/acs.analchem.9b05738

Figure Lengend Snippet: pH of droplets containing either control (grey) or hypoxia treated (orange) MDA-MB 231 cells. Square markers annotate unselected, circular markers selected droplets. pH values were determined via a fluorescence intensity ratio. A) Droplets presented as control and hypoxia cell populations. Average pH values, indicated by a black line, are 7.14 ± 0.02 (N = 66) for control and 6.95 ± 0.02 (N = 54) for hypoxia cells. B) Droplets presented as unselected and selected populations. Blue line indicates the pH of empty droplets (pH 7.37 ± 0.02). The pH threshold of selection was determined by a logistic regression fit and was found to be 6.96 ± 0.02 (threshold ± 95% confidence interval) (fit shown in Figure S5a). The sorting of droplets leads to an enrichment of hypoxia treated cells from 45 % (N = 54) before sorting to 75 % (N = 29) of selected cells.

Article Snippet: 42 Images and videos were taken on an inverted fluorescence microscope (Olympus IX-51) equipped with a 4X objective, equipped with a shuttered LED fluorescence excitation source (Spectra-X light engine, Lumencor, Beaverton, OR) and a high-speed camera (VEO-410, Vision Research, Wayne, NJ).

Techniques: Control, Fluorescence, Selection

Journal: Micromachines

Article Title: Method for Passive Droplet Sorting after Photo-Tagging

doi: 10.3390/mi11110964

Figure Lengend Snippet: Linear array of droplets with increasing irradiation times from left to right. ( A ) Fluorescence from blue excitation (436 nm). ( B ) Fluorescence from violet excitation (395 nm). pH of droplets as determined from blue to violet fluorescence intensity ratio based on calibration curve as shown in . ( C ) Droplet pH with increasing irradiation time with fit to a linear regression.

Article Snippet: Images and videos were taken on an inverted fluorescence microscope (Olympus IX-51) with a shuttered LED fluorescence excitation source (Spectra-X light engine, Lumencor, Beaverton, OR, USA) and a high-speed camera (VEO-410, Vision Research, Wayne, NJ, USA).

Techniques: Irradiation, Fluorescence

Journal: Micromachines

Article Title: Method for Passive Droplet Sorting after Photo-Tagging

doi: 10.3390/mi11110964

Figure Lengend Snippet: ( A ) Array device channel geometry. The location of the droplet array is highlighted by a dashed red square. ( B ) Bright-field image of droplet array. All wells are occupied with droplets that are approximately the same size as the wells. ( C ) Fluorescence from blue excitation. Irradiated droplets have lower fluorescence intensity. ( D ) Droplet array after the elution of non-irradiated droplets. Fluorescence excitation light was used in combination with bright-field image to increase droplet visibility.

Article Snippet: Images and videos were taken on an inverted fluorescence microscope (Olympus IX-51) with a shuttered LED fluorescence excitation source (Spectra-X light engine, Lumencor, Beaverton, OR, USA) and a high-speed camera (VEO-410, Vision Research, Wayne, NJ, USA).

Techniques: Fluorescence, Irradiation

Journal: Micromachines

Article Title: Method for Passive Droplet Sorting after Photo-Tagging

doi: 10.3390/mi11110964

Figure Lengend Snippet: ( A ) Bright-field image of droplet array. Droplets containing cells were classified as viable (green circle), early apoptosis (yellow diamond), late apoptosis (red square), and necrosis (black square). ( B ) Fluorescence image with blue excitation. The droplet with an early apoptosis cell was irradiated and displays lower fluorescence intensity. ( C ) Droplet array after the elution of non-irradiated droplets. The only droplet remaining in the array contains the early apoptosis cell.

Article Snippet: Images and videos were taken on an inverted fluorescence microscope (Olympus IX-51) with a shuttered LED fluorescence excitation source (Spectra-X light engine, Lumencor, Beaverton, OR, USA) and a high-speed camera (VEO-410, Vision Research, Wayne, NJ, USA).

Techniques: Fluorescence, Irradiation